ANTI-TUBULIN hFAB™ RHODAMINE ANTIBODY 12004165 from Bio-Rad (Formerly AbD Serotec)

Product Specs
ItemANTI-TUBULIN hFAB™ RHODAMINE ANTIBODY
CompanyBio-Rad
Price Supplier Page View Company Product Page
Catalog Number12004165
Quantity200 µl
ApplicationsWestern Blotting
Conjugate/TagRhodamine
ReactivityMouse, Human
TargetTUBULIN
CloneAbD22584
HostHuman
NCBI Full Gene Nametubulin beta class I
NCBI Gene AliasesOK/SW-cl.56, CDCBM6, CSCSC1, TUBB5, TUBB1, M40
ClonalityMonoclonal Antibody
IsotypeHuCAL Fab monovalent

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Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

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Website: http://discover.bio-rad.com

Website: Bio-Rad Life Science Research Product Promotions

(1) An siRNA library screen identifies CYLD and USP34 as deubiquitinases that regulate GPCR-p38 MAPK signaling and distinct inflammatory responsesJournal of Biological ChemistryDecember 1, 2023Norton Cheng, JoAnn Trejoanti-mouse (#12005867), StarBright Blue 700 goat anti-rabbit (#12004162), and hFAB Rhodamine Tubulin (#12004166) were from Bio-Rad. Vinculin antibody (#V9131) and SB203580 (#S8307) were from Sigma-Aldrich. Cell culture

(2) Genetic deficiency of ribosomal rescue factor HBS1L causes retinal dystrophy associated with Pelota and EDF1 depletionbioRxivOctober 26, 2023Shiyu Luo, Bilal Alwattar, Qifei Li, Kiran Bora, Alexandra K. Blomfield, Jasmine Lin, Anne Fulton, Jing Chen, Pankaj B. Agrawal(12419–1-AP, Proteintech, 1:500 dilution). Quantification of protein levels normalized to β-tubulin (12004165, Bio-Rad, 1:4000 dilution) was performed using Image J software. Statistical analysis. Data were analyzed

(3) Impaired Plakophilin-2 in obesity breaks cell cycle dynamics to breed adipocyte senescenceNature communicationsAugust 22, 2023Lluch A, Latorre J, Serena-Maione A, Espadas I, Caballano-Infantes E, Moreno-Navarrete JM, Oliveras-Cañellas N, Ricart W, Malagón MM, Martin-Montalvo A, et al.antibodies. Other antibodies used in this study include hFAB™ Rhodamine Anti-Tubulin Primary Antibody (#12004166) from Bio-Rad, rabbit anti-FAS (sc-20140) from SCBT, anti-PAPPA (AF2487) from R&D Systems, and anti-phospho-p38 MAPK (Thr180/Tyr182

(4) Divergent regulation of α-arrestin ARRDC3 function by ubiquitinationMolecular Biology of the CellAugust 1, 2023Helen Wedegaertner, Oye Bosompra, Irina Kufareva, JoAnn Trejofrom Thermo Fisher Scientific (Waltham, MA). Anti-tubulin hFAB conjugated to rhodamine (catalogue no.12004166) and goat anti-mouse (catalogue no. 170-6516) and goat anti-rabbit (catalogue no. 170-6515) secondary

(5) MLX plays a key role in lipid and glucose metabolism in humans: Evidence from in vitro and in vivo studiesMetabolismJuly 1, 2023Shilpa R. Nagarajan, Eilidh J. Livingstone, Thomas Monfeuga, Lara C. Lewis, Shahul Hameed Liyakath Ali, Anandhakumar Chandran, David J. Dearlove, Matt J. Neville, Lingyan Chen, Cyrielle Maroteau, et al.IB21001). Blots were probed with anti-MLX (1:1000; CST D8G6W), rhodamine anti-tubulin (1:1000; Bio-rad 12004166) and goat anti-rabbit (1:10000; Invitrogen 65-6120) antibodies and then imaged using Chemi-doc (Bio-Rad). 2.11

(6) Combinatorial selective ER-phagy remodels the ER during neurogenesisbioRxiv : the preprint server for biologyJune 26, 2023Hoyer MJ, Smith IR, Paoli JC, Jiang Y, Paulo JA, Harper JW.Polyclonal Antibody Proteintech (14643-1-AP, RRID:AB_2178440); hFAB™ Rhodamine Anti-Tubulin Antibody BioRad (12004166, RRID:AB_2884950); HSP90 mouse monoclonal Antibody Proteintech (60318, RRID:AB_2881429); Anti-Keima-Red mAb

(7) Interactions between TULP3 tubby domain and ARL13B amphipathic helix promote lipidated protein transport to ciliaMolecular biology of the cellMarch 1, 2023Palicharla VR, Hwang SH, Somatilaka BN, Legué E, Shimada IS, Familiari NE, Tran VM, Woodruff JB, Liem KF Jr, Mukhopadhyay S.Proteintech; 17711-1-AP), GFP (IF, Abcam ab13970; WB, Abcam ab290), hFAB rhodamine anti-tubulin (Bio-Rad; 12004166), acetylated α-tubulin (mAb 6-11B-1 T6793; Sigma-Aldrich), S tag (EMD Millipore; MAC112), IRDye 680RD

(8) Human IFT-A complex structures provide molecular insights into ciliary transportCell ResearchFebruary 13, 2023Meiqin Jiang, Vivek Reddy Palicharla, Darcie Miller, Sun-Hee Hwang, Hanwen Zhu, Patricia Hixson, Saikat Mukhopadhyay, Ji Sunfollowed by visualization using IRDye-tagged secondary antibody and hFAB Rhodamine Anti-Tubulin (Bio-Rad; 12004166). Images were taken in a BioRad Chemidoc MP imaging system. Statistical analyses Statistical analyses

(9) Development and characterization of a new monoclonal antibody against SARS-CoV-2 NSP12 (RdRp)Journal of medical virologyJanuary 1, 2023Meng W, Guo S, Cao S, Shuda M, Robinson-McCarthy LR, McCarthy KR, Shuda Y, Paniz Mondolfi AE, Bryce C, Grimes Z, et al.Invitrogen), SARS‐CoV‐2 Spike S2 (1:1000, NB100‐56578; Novus Biologicals), β‐tubulin rhodamine (1:10 000, 12004166; BioRad). Secondary antibodies included Goat anti‐Rat IgG (H + L) Highly Cross‐Adsorbed Secondary Antibody,

(10) 20 Years with SGBS cells - a versatile in vitro model of human adipocyte biologyInternational journal of obesity (2005)November 1, 2022Tews D, Brenner RE, Siebert R, Debatin KM, Fischer-Posovszky P, Wabitsch M.(Cell Signaling Technology #2443), rabbit anti-adiponectin (GeneTex #GTX112777), hFAB rhodamine anti-Tubulin (BioRad #12004165). Cellular flux analysis Cells were plated in 96-well cell culture microplates (XFe96, Agilent Technologies,

(11) Quantification of Genome Editing and Transcriptional Control Capabilities Reveals Hierarchies among Diverse CRISPR/Cas Systems in Human CellsACS Synthetic BiologyOctober 21, 2022Mario Escobar, Jing Li, Aditi Patel, Shizhe Liu, Qi Xu, Isaac B. HiltonBio-Rad, 1705062). Tubulin was detected with a human α-Tubulin Rhodamine-conjugated antibody (Bio-Rad, 12004165) at a 1:3000 dilution in Tris Buffered Saline With 1% Casein (Bio-Rad, 1610782). Intracellular Staining

(12) mTOR Contributes to the Proteome Diversity through Transcriptome-Wide Alternative SplicingInternational Journal of Molecular SciencesOctober 17, 2022Sze Cheng, Naima Ahmed Fahmi, Meeyeon Park, Jiao Sun, Kaitlyn Thao, Hsin-Sung Yeh, Wei Zhang, Jeongsik YongCell Signaling Technology), anti-S6 (sc-74576, Santa Cruz Biotechnology, Dallas, TX, USA), anti-Tubulin (12004166, Bio-Rad, Hercules, CA, USA), Anti-Secondary antibody against mouse: goat-anti-mouse IgG-HRP (No. sc-2005,

(13) Lineage-coupled clonal capture identifies clonal evolution mechanisms and vulnerabilities of BRAFV600E inhibition resistance in melanomaCell DiscoveryOctober 6, 2022Ze-Yan Zhang, Yingwen Ding, Ravesanker Ezhilarasan, Tenzin Lhakhang, Qianghu Wang, Jie Yang, Aram S. Modrek, Hua Zhang, Aristotelis Tsirigos, Andrew Futreal, et al.(Cat# 2367) and anti-Bcl-2 (Cat# 4223) were from Cell Signaling Technology, and anti-tubulin (Cat# 12004166) was from BioRad. The results were developed using ChemiDoc MP Imaging System (BioRad) and quantified

(14) Systematic comparison of CRISPR-based transcriptional activators uncovers gene-regulatory features of enhancer–promoter interactionsNucleic Acids ResearchAugust 12, 2022Kaiyuan Wang, Mario Escobar, Jing Li, Barun Mahata, Jacob Goell, Spencer Shah, Madeleine Cluck, Isaac B HiltonTrans-Blot turbo transfer system (Bio-Rad 1704150). Primary antibodies (anti-Cas9: Diagenode C15200216, anti-tubulin: Bio-Rad, 12004166) were used at a 1:1000 dilution in 1× TBS with 1% Casein. Secondary anti-mouse StarBright blue (Bio-Rad,

(15) Impact of DYRK1A Expression on TNNT2 Splicing and Daunorubicin Toxicity in Human iPSC-Derived CardiomyocytesCardiovascular ToxicologyAugust 1, 2022Romina Beatriz Cejas, Miriam Tamaño-Blanco, John Edgar Fontecha, Javier Guillermo BlancoIgG secondary antibody (1:2500, 12005869, Bio-Rad) and hFAB rhodamine anti-tubulin antibody (1:2500, 12004165, Bio-Rad) for 1 h at room temperature. Immunoreactive bands were visualized in a ChemiDoc MP gel imaging

(16) The CIP2A-TOPBP1 complex safeguards chromosomal stability during mitosisNature communicationsJuly 16, 2022De Marco Zompit M, Esteban MT, Mooser C, Adam S, Rossi SE, Jeanrenaud A, Leimbacher PA, Fink D, Shorrocks AK, Blackford AN, et al.Atlas Antibodies, HPA042933, 1:1500), Tubulin (Mouse, Sigma, T6199, 1:5000), Tubulin hFAB™ rhodamine (Bio-Rad, 12004166, 1:5000), FLAG M2 (Mouse, Sigma, F1804, 1:1000), pSer10 Histone H3 (Rabbit, Millipore, 06-570, 1:1000),

(17) MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cellseLifeApril 19, 2022Saito Y, Santosa V, Ishiguro KI, Kanemaki MT.IF" MCM3,Proteintech,15597-1-AP,IB,1:5000 MCM4,Abcam,ab4459,IB,1:5000 MCM5,Proteintech,11703-1-AP,IB,1:3000 MCM6,Proteintech,13347-2-AP,IB,1:5000 MCM7,Proteintech,11225-1-AP,IB,1:5000 MCMBP,Proteintech,19573-1-AP,IB,1:5000 FLAG,SIGMA,F1804,IB,"1:10,000" HA,MBL,M132-3,IB,1:5000 HSP90,Proteintech,"60,318",IB,1:5000 Tubulin,BioRad,12004165,IB,"1:10,000" histone H2B,Abcam,ab1790,IB,"1:10,000" p53,MBL,K0181-3,IB,1:5000 p21,CST,2946,IB,1:3000 gH2AX,Millipore,05-636,IB,1:3000 anti-rabbit

(18) Lipid flippase dysfunction as a therapeutic target for endosomal anomalies in Alzheimer's diseaseiScienceMarch 18, 2022Kaneshiro N, Komai M, Imaoka R, Ikeda A, Kamikubo Y, Saito T, Saido TC, Tomita T, Hashimoto T, Iwatsubo T, et al.antibody,Wako Pure Chemical Industries,Cat# 017-25031 anti α-tubulin hFAB Rhodamine antibody,Bio-Rad,Cat# 12004166; RRID: AB_2884950 APP-C terminal (C15) antibody,gift from Dr. T. Sakurai,Takasugi et al. (2018) TMEM30A-C

(19) Compound heterozygous variants in OTULIN are associated with fulminant atypical late-onset ORASEMBO molecular medicineMarch 7, 2022Zinngrebe J, Moepps B, Monecke T, Gierschik P, Schlichtig F, Barth TFE, Strauß G, Boldrin E, Posovszky C, Schulz A, et al.MCL‐1 (94296, rabbit, CST, dilution 1:1,000), Tubulin (2148, rabbit, CST, dilution 1:1,000), Tubulin (12004166, Rhodamine‐labelled, Bio‐Rad, dilution 1:5,000), OTULIN (14127, rabbit, CST, dilution 1:2,000), OTULIN

(20) Amyloid-like aggregating proteins cause lysosomal defects in neurons via gain-of-function toxicityLife Science AllianceMarch 1, 2022Irene Riera-Tur et al.Cell Signaling Technology), anti-p62 (ab56416; Abcam), anti-GFP (632381; Clontech), hFAB rhodamine anti-tubulin (12004166; Bio-Rad), anti-myc 9E10 (13-2500; Thermo Fisher Scientific), anti-Atg5 (D5F5U; Cell Signaling Technology),

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ANTI-TUBULIN hFAB™ RHODAMINE ANTIBODY

ANTI-TUBULIN hFAB™ RHODAMINE ANTIBODY

ANTI-TUBULIN hFAB™ RHODAMINE ANTIBODY

ANTI-TUBULIN hFAB™ RHODAMINE ANTIBODY from Bio-Rad (Formerly AbD Serotec)

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Bio-Rad

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ANTI-TUBULIN hFAB™ RHODAMINE ANTIBODY from Bio-Rad (Formerly AbD Serotec)

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